You can use replace
to replace unwanted characters in your string with an empty string:
'#[Header]'.replace('#', '') # '[Header]'
To do this in all strings in the list, just use a list understanding to apply replace
to all elements:
minha_lista = ['#[Header]', '#Application Name\tLCsolution', '#Version\t1.24']
minha_lista = [s.replace('#', '') for s in minha_lista]
Or do the same for just one slice:
minha_lista = ['#[Header]', '#Application Name\tLCsolution', '#Version\t1.24']
minha_lista[:2] = [s.replace('#', '') for s in minha_lista[:2]]