One way that I find simple is to use apply
import pandas as pd
df = pd.DataFrame({'col1':pd.date_range('2015-01-02 15:00:07', periods=3),
'col2':pd.date_range('2015-05-02 15:00:07', periods=3),
'col3':pd.date_range('2015-04-02 15:00:07', periods=3),
'col4':pd.date_range('2015-09-02 15:00:07', periods=3),
'col5':[5,3,6],
'col6':['10.000,00','10.000,00','10.000,00']})
df['col6'] = df['col6'].apply(lambda x: float(x.replace(".","").replace(",",".")))
print(df)
output
col1 col2 col3 \
0 2015-01-02 15:00:07 2015-05-02 15:00:07 2015-04-02 15:00:07
1 2015-01-03 15:00:07 2015-05-03 15:00:07 2015-04-03 15:00:07
2 2015-01-04 15:00:07 2015-05-04 15:00:07 2015-04-04 15:00:07
col4 col5 col6
0 2015-09-02 15:00:07 5 10000.0
1 2015-09-03 15:00:07 3 10000.0
2 2015-09-04 15:00:07 6 10000.0