neemias @ debian: / $ python
Python 2.7.9 (default, Aug 13 2016, 16:41:35)
[GCC 4.9.2] on linux2
Type "help", "copyright", "credits" or "license" for more information.
import Bio
from Bio.Emboss.Applications import Primer3Commandline
from Bio.Emboss import Primer3
cl = First3Commandline ()
cl.set_parameter ("- sequence", "/home/neemias/Documents/google_drive/agronomy/python/seq1.fasta")
cl.set_parameter ("- outfile", "/home/neemias/Documents/google_drive/agronomy/python/out.pr3")
cl.set_parameter ("- numreturn", 1)
First3Commandline (cmd = 'eprimer3', outfile = '/ home / neemias / Documents / google_drive / agronomy / python / out.pr3', sequence = '/ home / neemias / Documents / google_drive / agronomy / python / seq1. fasta ', numreturn = 1)
print cl
eprimer3 -outfile = / home / neemias / Documents / google_drive / agronomy / python / out.pr3 -sequence = / home / neemias / Documents / google_drive / agronomy / python / seq1.fasta -numreturn = 1
import them
os.system (str (cl))
Pick PCR primers and hybridization oligos
Error: Missing SEQUENCE tag
0
quit ()
(DOES NOT WORK ON PYTHON)
neemias @ debian: / $ eprimer3 -outfile = / home / neemias / Documents / google_drive / agronomy / python / out.pr3 -sequence = / home / neemias / Documents / google_drive / agronomy / python / seq1.fasta -numreturn = 1
Pick PCR primers and hybridization oligos
Error: Missing SEQUENCE tag
(DOES NOT WORK ON THE TERMINAL)
neemias @ debian: / $ EMBOSS_PRIMER32_CORE = / usr / bin / primer3_core eprimer32 -sequence /home/neemias/Documents/google_drive/agronomia/python/seq1.fasta -stdout / home / neemias / Documents / google_drive / agronomy / python /primer.out
Pick PCR primers and hybridization oligos
(WORKS AT TERMINAL)