Problems making Arduino serial connection with PHP

I'm trying to make a thermometer with PHP in Windows 10, and I'm capturing the data through an Arduino with the code:

#include <OneWire.h>

#include <DallasTemperature.h>

// Conectar o pino central dos sensores ao pino 10 do Arduino
#define ONE_WIRE_BUS 10

// Setup a oneWire instance to communicate with any OneWire devices
OneWire oneWire(ONE_WIRE_BUS);

// Pass our oneWire reference to Dallas Temperature. 
DallasTemperature sensors(&oneWire);
DeviceAddress thermometerOne = { 0x28, 0xFF, 0x72, 0xC0, 0x62, 0x15, 0x01, 0x38 };
DeviceAddress thermometerTwo = { 0x28, 0xFF, 0xFA, 0x65, 0x72, 0x15, 0x02, 0x4D };

void setup(void)
{
  // start serial port
  Serial.begin(9600);
  // Start up the library
  sensors.begin();
  // set the resolution to 10 bit (good enough?)
  sensors.setResolution(thermometerOne, 12);
  sensors.setResolution(thermometerTwo, 12);
}

void getTemp(){
  printTemperature(thermometerOne);
  Serial.print(" ");
  printTemperature(thermometerTwo);
}

void printTemperature(DeviceAddress deviceAddress)
{ 
  sensors.requestTemperatures();
  float tempC = sensors.getTempC(deviceAddress);
  if (tempC == -127.00) 
  {
    Serial.print("Erro");
  } 
  else 
  {
    Serial.print(tempC, 4);
  }
}

void IDFuncao(String funcao){
 if (funcao == String("temp")){
  getTemp(); 
 }
}

void decodificaMensagem(String mensagem){
  int i;
  String funcao = "";
  for (i=1; i<=4; i++){
    funcao = funcao + String(mensagem[i]);
  }
  IDFuncao(funcao);
}

void loop(void)
{ 
  //Verificando se tem algo na Porta
  if (Serial.available() > 0){
    String mensagem = Serial.readString();
    String inicio = String(mensagem[0]);

    //Verificando se o que eu recebi começa com #
    if (inicio == String("#")){
      decodificaMensagem(mensagem);
    }
  }
}

I have already researched several ways to capture data in PHP like this using phpserial: PhP Serial

include 'PhpSerial.php';

$serial = new PhpSerial;

$serial->deviceSet("COM5");
$serial->confBaudRate(9600);
$serial->confParity("none");
$serial->confCharacterLength(8);
$serial->confStopBits(1);
$serial->confFlowControl("none");    

$serial->deviceOpen();

sleep (1);
$serial->sendMessage("#");

global $ler_serial;
$ler_serial = $serial->readPort();

$serial->deviceClose();

however, I get this message:

  Warning: Unable to open the device [...]

And doing:

$port = "COM5";
$fp = fopen($port, "r+b");
//sleep(1);
fwrite($fp,'#');
print_r($fp);
print_r(fgets($fp));
fclose($fp);

I get:

  

Warning: fopen (COM5): failed to open stream: Permission denied

A friend of mine who did the Arduino code, he said that I needed to send a '#' to the Arduino, as if it were a protocol, sending the '#', or not, the result is the same. p>

What can I do to receive the temperatures captured by Arduino?