After creating the hosted zone, with the name of your domain, create CNAME and A type entries for the PUBLIC DNS and IP of your VM, for example:
NAME TYPE VALUE
seudominio.com.br A 192.168.0.128
www.seudominio.com.br CNAME ec2-xx-xx-xx-xx.us-west-2.compute.amazonaws.com
The public DNS and IP you get on the EC2 panel, right after you have created the VM
Now inside the VM, it is necessary of course to create a web environment, you can opt for nginx or apache, hence already another question ....
Also do not forget to change the DNS of your domain, to the DNS addresses created by route53, and wait for the propagation, which can take up to 24h.