Yes, it increases. So what?
First, it will be a minimal increase. Irrelevant in most, if not all cases. The gain will only be in the load. And you probably can not even measure it.
You probably do not need to have that gain. And if you need, there are many other things that are more important to improve performance.
Forget this, think about the organization.
It's obvious that the less disk access, the better. But if this is used little makes no difference, if it is used a lot it will already be in the operating system cache and the difference will be close to zero.
You'll have better benefit if you use a default language code caching system or third-party .
I do not know exactly what you're doing, but it's probably all in one file because it was not meant for a human to read, it does not have to be organized, so it's easier to do that. It does not put everything together because of the performance.